Journal: eLife

Article Title: Identifying prostate cancer and its clinical risk in asymptomatic men using machine learning of high dimensional peripheral blood flow cytometric natural killer cell subset phenotyping data

doi: 10.7554/eLife.50936

Figure Lengend Snippet: Descriptive statistics of the dataset.

Article Snippet: Antibody , Monoclonal mouse IgG2b anti human CD85j (ILT2) (clone GHI/75); FITC , Miltenyi Biotec , 130-098-437 , 10 μl per tube / 10 6 cells.

Techniques:

Journal: eLife

Article Title: Identifying prostate cancer and its clinical risk in asymptomatic men using machine learning of high dimensional peripheral blood flow cytometric natural killer cell subset phenotyping data

doi: 10.7554/eLife.50936

Figure Lengend Snippet: Antibody panels for measuring the phenotype of Natural Killer cells.

Article Snippet: Antibody , Monoclonal mouse IgG2b anti human CD85j (ILT2) (clone GHI/75); FITC , Miltenyi Biotec , 130-098-437 , 10 μl per tube / 10 6 cells.

Techniques:

Journal: eLife

Article Title: Identifying prostate cancer and its clinical risk in asymptomatic men using machine learning of high dimensional peripheral blood flow cytometric natural killer cell subset phenotyping data

doi: 10.7554/eLife.50936

Figure Lengend Snippet:

Article Snippet: Antibody , Monoclonal mouse IgG2b anti human CD85j (ILT2) (clone GHI/75); FITC , Miltenyi Biotec , 130-098-437 , 10 μl per tube / 10 6 cells.

Techniques: Staining, Saline, Software

Journal: iScience

Article Title: Defects in NK cell immunity of pediatric cancer patients revealed by deep immune profiling

doi: 10.1016/j.isci.2024.110837

Figure Lengend Snippet:

Article Snippet: Anti-ILT2 (clone GHI/75, conjugated to 156Gd) , Standard BioTools , Cat#3156020B.

Techniques: Purification, Clinical Proteomics, Recombinant, Blocking Assay, Staining, Saline, Mass Cytometry, Software, Cytometry

Journal:

Article Title: CD85/LIR-1/ILT2 and CD152 (Cytotoxic T Lymphocyte Antigen 4) Inhibitory Molecules Down-Regulate the Cytolytic Activity of Human CD4 + T-Cell Clones Specific for Mycobacterium tuberculosis

doi: 10.1128/IAI.69.10.6022-6029.2001

Figure Lengend Snippet: CD85/LIR-1/ILT2 and CD152 are expressed by M. tuberculosis-specific cytolytic CD4+ T-cell clones. CD85/LIR-1/ILT2 and CD152 surface and cytoplasmic immunofluorescence histograms of clones AM1 and FM1. CD85/LIR-1/ILT2 is detected by the M402 MAb on the surface and by the M402 (data not shown) and HP-F1 MAbs in the cytoplasm. CD152 shows similar behavior. Dotted lines represent negative controls obtained with isotype-matched irrelevant MAbs.

Article Snippet: The anti-CD85/LIR-1/ILT2 MAbs used for immunostaining were M402 (γ1; Immunex, Co., Seattle, Wash.), anti-CD85 (clone GHI/75; Pharmingen), and HP-F1 (provided by Miguel López-Botet).

Techniques: Clone Assay, Immunofluorescence

Journal:

Article Title: CD85/LIR-1/ILT2 and CD152 (Cytotoxic T Lymphocyte Antigen 4) Inhibitory Molecules Down-Regulate the Cytolytic Activity of Human CD4 + T-Cell Clones Specific for Mycobacterium tuberculosis

doi: 10.1128/IAI.69.10.6022-6029.2001

Figure Lengend Snippet: CD85/LIR-1/ILT2 and CD152 inhibit CD3-mediated activation of cytolytic CD4 + T-cell clones in redirected killing assays

Article Snippet: The anti-CD85/LIR-1/ILT2 MAbs used for immunostaining were M402 (γ1; Immunex, Co., Seattle, Wash.), anti-CD85 (clone GHI/75; Pharmingen), and HP-F1 (provided by Miguel López-Botet).

Techniques: Activation Assay, Clone Assay, Lysis

Journal:

Article Title: CD85/LIR-1/ILT2 and CD152 (Cytotoxic T Lymphocyte Antigen 4) Inhibitory Molecules Down-Regulate the Cytolytic Activity of Human CD4 + T-Cell Clones Specific for Mycobacterium tuberculosis

doi: 10.1128/IAI.69.10.6022-6029.2001

Figure Lengend Snippet: CD85/LIR-1/ILT2 and CD152 regulate antigen (Ag)-specific proliferation of cytolytic CD4+ T-cell clones. T-cell clones were restimulated by autologous irradiated PBMCs pulsed with PPD, in the absence or presence of soluble MAbs, alone or cross-linked by GAM antiserum. Four days later, proliferation was measured by [3H]thymidine uptake. Controls were provided by cells cultured with autologous PBMCs not pulsed with PPD, as well as by cells restimulated by PPD-pulsed PBMCs in the presence of GAM antiserum alone or in the presence of two irrelevant MAbs used as controls (i.e., anti-CD8 MAb or anti-MHC class I MAb). Asterisks indicate values that are significantly different for PPD, PPD plus HP-F1, PPD plus HP-F1 plus GAM, PPD plus anti-CD152, and PPD plus anti-CD152 plus GAM culture conditions. P < 0.05. ▨, blocking MAb conditions; private-char pc1, cross-linking of MAb specific for the inhibitory receptors.

Article Snippet: The anti-CD85/LIR-1/ILT2 MAbs used for immunostaining were M402 (γ1; Immunex, Co., Seattle, Wash.), anti-CD85 (clone GHI/75; Pharmingen), and HP-F1 (provided by Miguel López-Botet).

Techniques: Clone Assay, Irradiation, Cell Culture, Blocking Assay

Journal:

Article Title: CD85/LIR-1/ILT2 and CD152 (Cytotoxic T Lymphocyte Antigen 4) Inhibitory Molecules Down-Regulate the Cytolytic Activity of Human CD4 + T-Cell Clones Specific for Mycobacterium tuberculosis

doi: 10.1128/IAI.69.10.6022-6029.2001

Figure Lengend Snippet: Cross-linked CD85/LIR-1/ILT2 and CD152 inhibit restimulation and induce delayed cell death. Flow cytometric DNA content analysis was performed on clone AM1 10 days after restimulation carried out in the absence or presence of HP-F1 or anti-CD152, soluble or cross-linked by GAM antiserum. The marker to the left of the G1 peak (sub-G1 region) accounts for apoptotic cells, and the marker to the right of the G1 peak indicates S + G2M cells expressed as a percentage of the viable cells (i.e., S+G2M/G1+ S + G2M).

Article Snippet: The anti-CD85/LIR-1/ILT2 MAbs used for immunostaining were M402 (γ1; Immunex, Co., Seattle, Wash.), anti-CD85 (clone GHI/75; Pharmingen), and HP-F1 (provided by Miguel López-Botet).

Techniques: Marker

Journal:

Article Title: CD85/LIR-1/ILT2 and CD152 (Cytotoxic T Lymphocyte Antigen 4) Inhibitory Molecules Down-Regulate the Cytolytic Activity of Human CD4 + T-Cell Clones Specific for Mycobacterium tuberculosis

doi: 10.1128/IAI.69.10.6022-6029.2001

Figure Lengend Snippet: CD85/LIR-1/ILT2 and CD152 regulate the antigen-specific cytotoxic activity of CD4+ T-cell clones. The function of cytolytic CD4+ T-cell clones against autologous antigen-presenting B-EBV cells or monocytes was assayed in a conventional 4-h 51Cr-release assay, carried out in the absence or presence of soluble MAbs, alone or cross-linked by GAM antiserum. Soluble HP-F1 or anti-CD152 MAb significantly increased specific lysis, while the same MAb cross-linked by GAM antiserum reduced lysis. Control samples were treated with GAM antiserum alone or two irrelevant MAbs (i.e., anti-CD8 or anti-MHC class I MAb). Asterisks indicate values that are significantly different for PPD, PPD plus HP-F1, PPD plus HP-F1 plus GAM, PPD plus anti-CD152, and PPD plus anti-CD152 plus GAM culture conditions. P < 0.05. ▨, blocking MAb conditions; private-char pc2, cross-linking of MAb specific for inhibitory receptors.

Article Snippet: The anti-CD85/LIR-1/ILT2 MAbs used for immunostaining were M402 (γ1; Immunex, Co., Seattle, Wash.), anti-CD85 (clone GHI/75; Pharmingen), and HP-F1 (provided by Miguel López-Botet).

Techniques: Activity Assay, Clone Assay, Release Assay, Lysis, Blocking Assay

Journal:

Article Title: CD85/LIR-1/ILT2 and CD152 (Cytotoxic T Lymphocyte Antigen 4) Inhibitory Molecules Down-Regulate the Cytolytic Activity of Human CD4 + T-Cell Clones Specific for Mycobacterium tuberculosis

doi: 10.1128/IAI.69.10.6022-6029.2001

Figure Lengend Snippet: CD85/LIR-1/ILT2 and CD152 down-regulate IL-2 and IFN-γ production by cytolytic CD4+ T-cell clones. Twenty-four hours after restimulation of CD4+ T-cell clones with OKT3 and irradiated feeder PBMCs, the amount of IL-2 and IFN-γ in the culture medium was measured and expressed as picograms per milliliter. At the time of restimulation, HP-F1 and anti-CD152 were added to the cultures alone (blockade of receptors) or cross-linked by GAM antiserum (cross-linking of the receptors). A negative “restimulation control” was provided by T cells exposed to irradiated autologous feeder PBMCs only, in the absence of OKT3. Asterisks indicate values that are significantly different for anti-CD3, anti-CD3 plus HP-F1, anti-CD3 plus HP-F1 plus GAM, anti-CD3 plus anti-CD152, and anti-CD3 plus anti-CD152 plus GAM culture conditions. P < 0.05.

Article Snippet: The anti-CD85/LIR-1/ILT2 MAbs used for immunostaining were M402 (γ1; Immunex, Co., Seattle, Wash.), anti-CD85 (clone GHI/75; Pharmingen), and HP-F1 (provided by Miguel López-Botet).

Techniques: Clone Assay, Irradiation